Southwest Research and Outreach Center

 

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Trial: 2002-SBAfoliar

Foliar applied insecticide control of the Soybean Aphid (Aphis glycines). DRAFT

Bruce Potter, F. Breitenbach, C. Holen, D. Noetzle, K. Ostlie, D. Ragsdale, University of Minnesota, Department of Entomology

 

Background

The soybean aphid (SBA) is a new pest of Minnesota soybeans. It was first identified during the late summer of 2000 in SE MN. SBA subsequently spread throughout the soybean growing areas of MN during the 2001 growing season. Little information on insecticide performance for this pest is available, especially under non-drought stressed conditions.

 

This trial was designed to look at insecticide performance of compounds in several insecticide classes (Pyrethroid, Organophosphate, Neonicotinoid). Additionally, high and low use rates were examined for at least one compound in each insecticide class.

 

Site and application information

The trial was placed in a production soybean field near New Ulm, MN. A stream with wooded margins containing buckthorn, Rhamnus sp., bordered the field. This field had one of the earliest detectable SBA populations in the area and populations had been steadily increasing. Initial aphid populations at insecticide application averaged 33 aphids per upper trifoliate or a rating of 7.25. These individual leaf populations translated to over 300 aphids/plant.  Soybean growth, however, was exceptional and showed no evidence of injury by SBA.  These populations should be considered moderate based on soybean stage at infestation and soybean growth.

 

Cooperators: Randall Reinhart, Steve Commerford

County: Nicollet, MN

Nearest town: New Ulm, MN

Soil type: Nicollet clay loam

Cultivar: Stine 1700-4. 

Row spacing: 30”

Planting date: May 6, 2002

Design: Randomized complete block with 4 replications

Plot size: 10’X30’

Application date: July 26,2002

Crop stage at application: R3

Crop height at application: 43 inches

Soybean aphid population at application: 33 aphids/uppermost expanded leaf (SBA rating 7.25)

 

Insecticide treatments were applied with a CO2 pressurized backpack sprayer, 8002XR flat fan nozzles on 18-inch spacing, 20 gallons/acre, 35 PSI.

 

 

Results and discussion

High winds and heavy rain occurred the evening of July 28,2002 or 2 days after treatment (DAT). This caused temporary soybean lodging and reduced aphid populations in untreated plots by 80% at the 4 DAT rating.  After this setback, SBA populations increased in untreated checks until 14 DAT.  SBA populations were declining in untreated checks by 21 DAT.  Individual treatment results are presented in Table 1 and Figure 1.

 

Soybean aphid populations were estimated by single leaf (trifoliate) samples from 20 plants/plot. The leaves selected for sampling were based on which part of the canopy had the highest aphid populations. SBA densities were initially greatest on the upper canopy. By 7DAT the greatest densities were located on mid canopy leaves. This coincided with end of new leaf production.  Percent SBA control data is based on raw counts of aphids/leaf compared to untreated checks. Data were transformed to a 1-10 scale (Table 1) to perform an analysis of variance (ANOVA) and means were separated by Duncan’s new multiple range test. 

 

Excellent aphid control was obtained with moderate water volume and pressure in spite of a tall, dense soybean canopy. All treatments had significantly less aphids than the untreated check for 4, 7and 15 DAT. Plots treated with Dimethoate 4E (56% control) and the neonicitinoids, Actara (74% control) and Assail 70WG (.025 #/A rate)(80% control), had greater aphid populations than other insecticides at 4 DAT. Based on 7 DAT ratings, aphid populations were significantly higher in plots treated with Actara and the low rate of Assail 70WG. However, all insecticide treatments averaged less than 1 aphid per leaf or greater than 99% control at this date. Dimethoate 4E and Actara had greater aphid populations (85% and 84% control respectively) than other insecticide treatments (>=98%control) at 15 DAT. Although not significant, there was a trend for lower aphid populations with the higher treatment rates for those products with rate comparisons. 

 

Treatment yields are presented in Table 2. Trial yields were good for Southern Minnesota soybean production with the trial averaging 61.2 bushels/acre. Yield differences were not observed between any of the treatments. Bean leaf beetle were abundant in the trial before insecticide application. Beetle populations were well controlled by insecticide application within and surrounding the trial. Evidence of bean leaf beetle feeding on pods was much less than 1% of pods. Sclerotinia infected 4.7% of the plants in the trial and symptoms of Colletotrichum were observed on nearly all plants. Additionally, symptoms of bean pod mottle virus, likely transmitted before insecticide application, and other disease were observed on seed.

 

The lack of yield response to aphid control raises several hypotheses: 1) The soybean aphid populations present were not great enough to impact yield in vigorously growing, late flowering and pod fill soybeans. 2) The storm event after insecticide application temporarily reduced populations enough to minimize impact of soybean aphid. 3) Other factors influenced yield to a greater extent than soybean aphid. Unfortunately, the data in this trial cannot test these hypotheses.

 

 

Acknowledgments: Thanks to Scott Anderson, Steve Commerford, Derek Erickson, Tim Lendt, and Phil Price for aphid count and application assistance


Table 1.         Foliar applied insecticide control of Soybean Aphid

Randall Reinhart farm, Nicollet County, MN  2002

Potter, Holen, Noetzle, Ostlie, U of M

Treatment Number

Product

Product Rate lbs. A.I./A

Product Rate Product/A

07/30/02
SBA rating1
4 DAT

08/02/02 SBA rating1 7 DAT

08/10/02 SBA rating1 15 DAT

1

Untreated

 

 

5.0 a

6.5 a

8.0 a

2

Dimethoate 4EC

0.500

1.0 pt.

3.5 b

1.8 cd

5.0 b

3

Lorsban 4E

0.500

1.0 pt.

1.0 e

1.5 d

2.0 c

4

Lorsban 4E

1.000

2.0 pt.

1.0 e

1.5 d

1.8 c

5

Warrior

0.015

1.92 fl. oz.

1.8 de

1.3 d

2.0 c

6

Warrior

0.025

3.20 fl. oz.

1.3 e

1.0 d

1.8 c

7

FO0570 0.08EW2

0.025

4.0 fl. oz.

2.0 cde

1.3 d

2.0 c

8

Asana XL

0.033

6.4 fl. oz.

2.0 cde

1.3 d

2.0 c

9

Asana XL

0.046

9.0 fl. oz.

2.0 cde

1.0 d

1.8 c

10

Assail 70WG2

0.025

0.57 oz.

2.8 bcd

2.3 bc

2.5 c

11

Assail 70WG2

0.050

1.14 oz.

1.8 de

1.3 d

2.3 c

12

Actara 70WG2

0.023

1.50 oz.

3.0 bc

2.5 b

5.5 b

13

Pounce 3.2 EC

0.200

8.0 fl. oz.

2.0 cde

1.8 cd

2.5 c

14

XDE2252

0.013

3.2 fl. oz.

1.3 e

1.3 d

2.5 c

15

Nudfos

0.500

1.0 pt.

1.8 de

1.3 d

2.0 c

 

LSD (p=0.05)

 

 

0.9

0.7

0.9

 

CV

 

 

29.4

25.6

22.5

 

Prob(F)

 

 

<0.01

<0.01

<0.01


 


Means within columns followed by the same letter(s) are not significantly different (p <0.05), least significant difference (LSD), Duncan’s New MRT.

 

1 Soybean aphid density was estimated by counting the number of aphids /trifoliate leaf from 20 randomly selected plants in the center two rows of each/plot. Leaves were selected from the portion of the canopy most heavily colonized at each sampling date. Aphid density was greatest on uppermost-expanded trifoliates at the pretreatment and 4 days after treatment (DAT) sampling and mid canopy leaves (terminal – 6 nodes) for 7 and 14 DAT sampling. 

 

Aphid counts/trifoliate leaf were transformed to the following scale for analysis of variance: 1) no SBA, 2) >0 -1 SBA, 3) >1-2 SBA, 4)>2-4 SBA, 5)>4-8 SBA, 6)>8-16 SBA, 7)>16-32 SBA, 8)>32-64 SBA, 9)>64-128 SBA, 10) >128 SBA.

 

2 Not currently labeled on soybeans

 


Table 2.         Yield response to soybean aphid control

Randall Reinhart farm, Nicollet County, MN  2002

Potter, Holen, Noetzle, Ostlie, U of M

 

Treatment Number

Product

Product Rate lbs. A.I./A

Product Rate Product/A

Bushels/acre@ 13% moisture

1

Untreated

 

 

58.7 a

2

Dimethoate 4EC

0.500

1.0 pt.

61.9 a

3

Lorsban 4E

0.500

1.0 pt.

61.1 a

4

Lorsban 4E

1.000

2.0 pt.

59.9 a

5

Warrior

0.015

1.92 fl. oz.

59.5 a

6

Warrior

0.025

3.20 fl. oz.

61.4 a

7

FO0570 0.08EW2

0.025

4.0 fl. oz.

63.4 a

8

Asana XL

0.033

6.4 fl. oz.

60.2 a

9

Asana XL

0.046

9.0 fl. oz.

60.9 a

10

Assail 70WG2

0.025

0.57 oz.

62.3a

11

Assail 70WG2

0.050

1.14 oz.

62.6 a

12

Actara 70WG2

0.023

1.50 oz.

61.2 a

13

Pounce 3.2 EC

0.200

8.0 fl. oz.

62.6 a

14

XDE2252

0.013

3.2 fl. oz.

61.9 a

15

Nudfos

0.500

1.0 pt.

60.9 a

 

LSD (p=0.05)